Factors Affecting Crime Scene Investigations
Overview
Objectives In crime scene investigations, it is easy to miss evidence of blood stains without careful application of Luminol, a chemiluminescent substance. My objective is to study the impact of various factors affecting the glow of Luminol. Methods Luminol, Perborate Mixture, Copper Sulfate Crystals, three cups, two small table spoons, Precision Thermometer, pH strips, Google Science Journal App. Each experiment involves three trials in a dark room. A vial of water with four Copper Sulfate crystals, either crushed up or dissolved, is poured into a different cup of water with Luminol and Perborate Mixture mixed into it. The variables being tested are temperature, acidity, larger amounts of Luminol, and crushed up Copper Sulfate vs crystals. The light and duration is measured in the Google Science Journal App, which measures the glow, and charts it on a data point graph and can be exported as a .CSV file. Results I observed that the larger amount of Luminol had the longest duration of luminescence. With changing the temperature, I observed that the Luminol mixture at 145 F had the brightest glow and was only five seconds long, while the Luminol mixture at 85 F was dimmer and slightly longer. A more acidic mixture showed absolutely no light in the graph, and the crushed up Copper Sulfate graph was fluctuating and inconsistent. Conclusions The results showed that between a higher temperature of water and a colder temperature of water, a colder temperature mixture of Luminol would be better, because it glows for a longer duration. Even though a hot mixture would be much brighter, it would only last a few seconds, so it becomes harder to capture as evidence. The results also showed that any trace of acidity would make the Luminol mixture not glow, and this shows that high levels of acidity should be avoided.
Summary statement
When finding how independant variables affect the brightness and the duration of the glow of Luminol, I established that a cold mixture with generous amounts of Luminol produces brighter and more sustained results.
Help received
My dad and my sister helped brainstorm ideas and potential variable. Mrs. Patel, the Windemere Ranch Middle School science fair advisor, also guided me through the scientific method.
Competition history
- CSEF 2019
Resources
Related projects
ISEF · 2014
The Effect of Surface Temperature on Chemiluminescence
CSEF · 2014
Luminol Reaction Times
CSEF · 2002
The Effect of Various Household Chemicals on Hemoglobin Testing with Luminol
ISEF · 2014
You Missed a Spot: Accuracy of Luminol Chemiluminescence to Detect Blood at a Crime Scene using Concealment Techniques and Measurements of False Positives
CSEF · 2009
Effect of Varying Oxidizing Agent in a Luminol Mixture on Chemiluminescence Time
CSEF · 2026
Blood, Blue, and Beyond
CSEF · 2015
SFI: Science Fair Investigation
CYSF · 2026
Crime Scene Chemistry Investigating The Cool Blue Light of Luminol
Closest projects by meaning, across every fair and year in the corpus.
Browse more like this
Source: California Science & Engineering Fair public projects