Biochemistry/ Molecular Biology
Objectives/Goals The purpose of this study was to investigate the effects a dominant-negative p53 protein has on the cellular and molecular response of prostate cancer cells to radiation. Th…
Biochemistry/ Molecular Biology
Objectives/Goals The blood brain barrier is an important structure in the maintenance of brain homeostasis. Protease nexin 1 (PN1) is a 43-kDa serine protease inhibitor (serpin) found predom…
Biochemistry/ Molecular Biology
Objectives/Goals Given three linked loci, yellow body (y), a rough eye known as echinus (ec), and cut wings (ct), what is their frequency of recombination and thus the distance between them,…
Biochemistry/ Molecular Biology
Objectives/Goals My objective is to better understand Alzheimer's disease through fluorescent immunohistochemistry and double labeling. 1) Establish the distribution of BDNF (brain-derived n…
Biochemistry/ Molecular Biology
Objectives/Goals The objective is to determine if plant pigments were distinctive for each species. Methods/Materials Leaves of different plants were obtained. Residue from leaves was applie…
Biochemistry/ Molecular Biology
Objectives/Goals Professor Mary Williams at Harvey Mudd College has identified a mutant in the plant Arabidopsis, and as a result of past experiments has concluded that it contains an F-Box…
Biochemistry/ Molecular Biology
Objectives/Goals The purpose of the ezperiment is to show the basic and at times advanced fucnctions of DNA such as replication, translation, transcription, through the study of DNA sections…
Biochemistry/ Molecular Biology
Objectives/Goals The objective was to determine if there is a difference between the characteristics of DNA extracted from white onions using laboratory reagents versus household materials.…
Biochemistry/ Molecular Biology
Objectives/Goals Investigations were performed on two sets of glioma tumors that were generated earlier in the absence or presence of Neurotrophin-3 (NT-3) with a significant difference in s…
Biochemistry/ Molecular Biology
Objectives/Goals Thymidylate synthase (TS) catalyzes the reaction transforming dUMP into dTMP in cells. In cancer cells, TS is expressed at an increased level. While usual drugs treat cancer…
Biochemistry/ Molecular Biology
Objectives/Goals The objective is to determine if temperature fluctuations in the environment will affect the rate of oxygen produced by the enzymatic activity of the enzyme catalase. Method…
Biochemistry/ Molecular Biology
Objectives/Goals The objective of this project was to accuratly test pears to see if placing them in different conditions would effect their ripening process. Methods/Materials 15 unripe pea…
Biochemistry/ Molecular Biology
Objectives/Goals To investigate the effect of ampicillin and kanamycin on the lactose operon induction of Escherichia coli through enzyme assays. Methods/Materials In the experiment, the pro…
Biochemistry/ Molecular Biology
Objectives/Goals The goal of my project was to detirmine how the Hfr-1 resistince gene is affected by the Hessian Fly. Methods/Materials Using TRIzol rna preps I was able to quatify wheat ti…
Biochemistry/ Molecular Biology
Objectives/Goals The goal of this study is to identify single amino acid mutations in the p53 gene of Drosophila melanogaster. Methods/Materials DNA from several fly strains, some with domin…
Biochemistry/ Molecular Biology
Objectives/Goals This study investigated the interaction between integrin a3/b1and its ligand, laminin-5 in modulating alveolar epithelial barrier. Methods/Materials Cell Culture and prepara…
Biochemistry/ Molecular Biology
Objectives/Goals Our purpose is to monitor the differences in gene expression between a cell line of pulmonary carcinoma (HTB174), a normal cell line (HTB175), and normal tissue using oligon…
Biochemistry/ Molecular Biology
Objectives/Goals Just like Dr. Frankenstein and his accomplice, Igor, I created mutants. Now, my mutants are a bit different from Frankenstein, but nonetheless they are paving a new path for…
Biochemistry/ Molecular Biology
Objectives/Goals How little DNA is necessary to amplify a gene by polymerase chain reaction (PCR)? My objective was to determine the optimal conditions for PCR to amplify the tubulin gene fr…
Biochemistry/ Molecular Biology
Objectives/Goals The ability to predict protein structure from amino acid sequence alone would be invaluable to scientists everywhere. A pharmacologist working on creating a new drug, for ex…
Biochemistry/ Molecular Biology
Objectives/Goals The objective of my project was to see if the pH of a porous gel had an effect on the the structure of DNA molecules that are running through it. Methods/Materials Gels were…
Biochemistry/ Molecular Biology
Objectives/Goals My project mainly focused in testing the effects of antioxidants in slowing the rate of oxidation in a TBA analysis using pure pork, beef, and vegetable oil. In the tests I…
Biochemistry/ Molecular Biology
Objectives/Goals My project was to determine if by specifically directing a ribozyme to attack the Proliferating Cell Nuclear Antigen (PCNA mRNA), will the growth of a cancer cell be inhibit…
Biochemistry/ Molecular Biology
Objectives/Goals I tested the effects of temperature on the enzyme peroxidase. Methods/Materials In the presence of hydrogen peroxide, peroxidase reacts to produce visible gas bubbles. I use…
Biochemistry/ Molecular Biology
Objectives/Goals The project objective was to determine if household chemicals other than detergent or bleach would interfere with the reaction of luminol and hemoglobin in a hypothetical cr…
Biochemistry/ Molecular Biology
Objectives/Goals The object of my project was to determine if I could extract pure DNA by a simple kitchen technique from several diverse plant and animal species, quantify the amount, and c…
Biochemistry/ Molecular Biology
Objectives/Goals The populace of Greater Los Angeles, a highly urbanized metropolis, use an abundance of chemicals every day. As a result, increased concentrations of endocrine disruptive co…
Biochemistry/ Molecular Biology
Objectives/Goals The purpose of this study is to determine some conditions that affect the reaction rate of the lactate dehydrogenase (LDH) enzyme. How does changing the amount of enzyme pre…
Biochemistry/ Molecular Biology
Objectives/Goals What affect dosetemperature variation has on fruit flies (Drosophila) Larva Cells, so that DNA could be extracted from them? How many times will be nesessary to isolate the…
Biochemistry/ Molecular Biology
Objectives/Goals I wanted to see which medicine would have the most effect on the patelet activity. Methods/Materials I used the Surgicutt Jr. bleeding time tester and blotting papers. I als…