Hfr-1 and the Hessian Fly

Overview

Objectives/Goals The goal of my project was to detirmine how the Hfr-1 resistince gene is affected by the Hessian Fly. Methods/Materials Using TRIzol rna preps I was able to quatify wheat tissue tht had been infested with Hessian Flys. Then I ran Northerns to move the rna onto a Hibond membrane. I then hibordized with a radioactive probe and exposed the membrane to film. TRIzol, chloroform, ethonol, isopropanol, rna precipitation solution, agarose/formaldahyde gel: various pipetts, sterolized tubes, gel boxes Results The results of my three expeiments were as follows: Exp. 1- I infested three pots with three male flys and three female flys, three pots with six of each sex, three pots with twelve of each sex,and three pots with twenty-four of each sex. Each group has its own uninfested control. I harvested the pots five days later. I performed TRIzol rna preps and Northerns to come up with my results. The Hfr-1 resistance gene is not affected by higher levels of Hessian Flys. Exp. 2 I wanted to see in which part of the wheat plant Hfr-1 was most induced. I infested ten pots with six flys of each sex. I then took parts of the plant. I detirmined that Hfr-1 is most induced 2cm above the ground. Exp. 3 I wanted to detirmine if as long as Hessian Flys were present that Hfr-1 would stay indced. I infested eight pots with six flys of each sex. I then harvesed one group and reinfestedthe others. I repeated this process until I finished them all. My results were that Hfr-1 is not affected by the amount of time the flys are peresent. Conclusions/Discussion Exp.1 The Hfr-1 resistance gene is not affected by large amounts of Hessian Flys. Exp.2 Hfr-1 is more heavily induced 2cm above the ground Exp. 3 Hfr-1 is not afeected any diferently as long as Hessian Flys are present.

Competition history

• CSEF 2002 Biochemistry/ Molecular Biology · Entry S0404

Resources

• CSEF project summary ↗

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