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Effect of Neurotrophin-3 on Suppression of Tumor Vascularization

CSEF · 2002 Biochemistry/ Molecular Biology

Anshu R. Abhat · Flintridge Preparatory School, La Canada Flintridge

Overview

Objectives/Goals Investigations were performed on two sets of glioma tumors that were generated earlier in the absence or presence of Neurotrophin-3 (NT-3) with a significant difference in size. The objective of my study was to evaluate and analyse the disparity between the growth of the two tumors. Methods/Materials Immunohistochemistry was performed on cryostat sections using antibodies specific for brain cell types and protein markers expressed on blood vessels. I further carried out double immunofluorescence imaging using different conjugated fluorochromes (red and green) with an epifluorescence microscope. Results The evaluation of digital images of AT and ATNT-3 glioma tumors revealed an elaborate network of vascularization (angiogenesis) of AT tumor and a total lack of angiogenesis in ATNT-3 tumor. The analysis further showed that progression of AT tumor growth appears to be directly related to the supply of growth nutrients in the core of the tumor mass. Conclusions/Discussion The present study suggests an inhibitory role of NT-3 in angiogenesis and suppression of glioma growth. NT-3 appears promising in tumor therapy.

Summary statement

The comparative study of the AT tumor and the ATNT-3 tumor suggests that NT-3 has the potential to prevent vascularization.

Help received

Mentor at UCLA (Dr. Shalini Kumar) provided the tissue samples, antibodies, and microscope for investigation. Lab facilities at UCLA were used. Father helped arrange poster board.

Awards (1)

  • Category Award

Competition history

  • CSEF 2002 Biochemistry/ Molecular Biology · Entry S0401

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