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Enzymatic Activity

CSEF · 2002 Biochemistry/ Molecular Biology

Katherine S. Wu · South Lake Middle School, Irvine

Overview

Objectives/Goals The objective is to determine if temperature fluctuations in the environment will affect the rate of oxygen produced by the enzymatic activity of the enzyme catalase. Methods/Materials My My main materials were porcine liver, test tubes, hydrogen peroxide, detergent, and oil. The temperature of the environment of liver in a testtube(which contains the enzyme catalase) was changed. One control had no liver in it.After one drop of oil, detergent, and then hydrogen peroxide were dropped into the test tube, the resulting foam was measured with a ruler. Results The enzymatic activity peaked consistently at 40 degrees Celcius throughout the three trials conducted. Conclusions/Discussion My conclusion is that temperature does have an effect on enzymatic activity, and that enzymatic activity peaks at forty degrees Celcius.

Summary statement

My project is focused on how temperature affects the rate of oxygen produced by enzymatic activity.

Help received

My teacher loaned materials for experiment and helped me with several difficulties in my project; my mom and dad took me to the library and printed out the color graphs at their companies.

Competition history

  • CSEF 2002 Biochemistry/ Molecular Biology · Entry J0406

Resources

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