Ivermectin Induces Apoptosis, Cell Cycle Arrest, and Senescence in C4-2 Prostate Cancer Cells.

Overview

Dr. Laura E. Pascal Dr. Zhou Wang UPMC Hillman Cancer Center Dr. Grant Yamashita Dr. Ali Seyedali Ms. Gail Ishimoto Kamehameha Schools Ivermectin, awarded the Nobel Prize f or Physiology or Medicine in 2015, was originally u sed as an anthelmintic. Ivermectin w as i nvented by William Campbell and Satoshi Omu ra to tre at parasites , mainly onch ocerciasis. Ivermectin h as b een FDA approve d for use in humans and h as no side effects on human s afety. Due to this , Ivermectin has been researched in multiple cancer cell lines and was fo und to have anti cancer activity in variou s types of cancer. This project focuses on the effect of Ivermectin on the C4-2 prostate cancer cell line, which has not been researched with Ivermectin. The C4-2 prostate cancer cell line is a human castration resistant cell line derived of the LNCaP cell line. Multiple experimental procedures were used to test the effect of Ivermectin on the C4-2 cell line. For all experimen ts C4-2 cells were treated with a DM SO control and various concentrations of Ivermectin. It was found that Ivermectin induces apoptosis, cell cycle arrest in the G1/G0, and cell senescence. Las tly, ivermectin was found to de crease the FOXA1 protien which is necessary f or AR bi nding to DN A, thus causing decreased AR expression and AR activity which is import ant in DNA damage repair. Ther efore, in creased DNA damage wa s detected in Ivermectin treated cells. The effect of Ivermectin on RPMI myeloma cells and HCT colorectal cancer cell lines was also tested. It was found that Ivermectin can decrease the ce ll density of RP MI myeloma ce lls without

Awards (1)

Poster Peer Awardee

Competition history

JSHS 2020 Category not listed

Resources

National JSHS abstract book

Related projects

ISEF · 2020