Testing the Efficacy of Mebendazole Induced Apoptosis in Pancreatic Cancer Cells

Overview

According to the American Society of Clinical Oncology (ASCO), Pancreatic Cancer is the fourth leading cause of cancer-related death and has a five-year survival rate of 8%.1 Previous research at John Hopkins and the National Institute of Health (NIH) has shown that Mebendazole2 has been an effective treatment for Brain3, Gastric4 and Colon5 Cancer. Research done last year, tested the effectiveness (measured by cell viability using trypan blue) of Mebendazole at a 1, 3, and 10µM dilutions on a Pancreatic Cancer Cell-line (ATCC UACC-462).6 The Pancreatic Cancer Cell-line is an epithelial tissue derived from the Peritoneal Metastases site, which is the primary site where the Pancreatic Cancer formed before spreading throughout the body. Peritoneal Metastases occurs in about 50% of Pancreatic Cancer patient at or before time of death according to the NIH.7 A healthy Pancreatic cell-line, obtained from Cell Biologics8, were also used to rule out any risk of contamination and to test the response of healthy Pancreatic cells when exposed to Mebendazole at the three concentrations. The study found that Mebendazole was effective at reducing cell viability significantly (P<0.05) at all three concentrations without greatly compromising the healthy Pancreatic cell-line. This year in partnership with Mote Marine Laboratory in Sarasota, Florida, the research will study the pathways of Apoptosis9, which is a programmed cell death that occurs in multicellular organisms.10 The research will use a caspase-3 assay11 to determine if Apoptosis is occurring in the Pancreatic Cancer Cell-line (ATCC UACC-462) causing the Pancreatic Cancer Cells to die off when exposed to Mebendazole at a 3µM dilution and measured using Cell Flow Cytometry methodology.12-16

Competition history

• AJAS 2019 Category not listed

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