fairfolio
Saved Projects

Impact of Klotho Gene Variability and DNA Methylation on Traumatic Brain Injury Outcomes

ISEF · 2020 Biomedical and Health Sciences

Naumov, Daniela · Pittsburgh Allderdice High School

Overview

Traumatic brain injury (TBI) affects about 1.7 million individuals annually. It is one of the leading causes of death and disability in the U.S. The Klotho gene, which plays an important role in normal cognitive function and longevity, may influence patient outcomes after TBI. It was hypothesized that DNA variability and DNA methylation within the Klotho gene would significantly impact TBI outcomes. Discovery data collection led to a follow-up allelic discrimination that assessed DNA variability. Group-based trajectory analyses were used to quantify DNA methylation. Regression analyses were run to relate DNA variability and methylation to TBI outcomes. The discovery analysis discovered one SNP, rs508394, that showed significance to death at 3 and 12 months after TBI (p=0.048, 0.016) and disability (p=0.046) at 3 months after TBI. While there was no significance observed between DNA methylation and TBI outcomes, the Klotho methylation trajectory plots provided insight into the genetic basis of recovery after TBI. Together, these findings may be important when evaluating Klotho as a potential biomarker for TBI outcomes, thereby furthering our knowledge on genetics-based therapies.

Competition history

Resources

Related projects

Closest projects by meaning, across every fair and year in the corpus.

Browse more like this

Source: Regeneron International Science and Engineering Fair

Save projects to your library

Sign in with Google to keep track of projects you find interesting, organized into folders. An account also raises your daily allowance for “Has this been done?”, and lets you create a key for the MCP server with a much higher limit than anonymous use. Browsing stays public.

Continue with Google