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Daphnia: Under the Influence

CSEF · 2015 Toxicology

Joshua A. Lewis · Pressman (Rabbi Jacob) Academy, Los Angeles, Los Angeles County

Overview

Objectives/Goals To find whether certain substances affect the heart rate of Cladocera Daphnia. Methods/Materials Water fleas, caffeine, alcohol, microscope. I added small amounts of alcohol to the container of Daphnia and then extracted them from the container after a minute with a pipet. I then put them under the microscope and counted their heartbeats for 15 minutes, then removed the Daphnia. I repeated this process multiple times to get a more accurate sample size. I repeated the process again multiple times, but with caffeine instead of alcohol. Results When the Daphnia were introduced to caffeine, the more I gave them, the more their heart rate went down. The more alcohol I gave the Daphnia, the more their heart rate went down.

Summary statement

Testing whether caffeine and alcohol affect Cladocera Daphnia's heart rate.

Help received

My mother helped time the experiments, and my dad bought the supplies. My math tutor helped me keep track of data.

Competition history

  • CSEF 2015 Toxicology · Entry J2105

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