Antioxidant Activities of Human Retinal Cell Lines under the Exposure of 2.4 GHz Electromagnetic Field

Overview

Objectives/Goals To see if the electromagnetic wave is a source of oxidative stress and the effects of it on antioxidant enzyme activities after the 2.4GHz exposure. Methods/Materials Method: In the experiment, retinal ganglion cell line and retinal pigment epithelium cell line were used as two eye cell models. Both cells were cultured separately so that we obtained enough number of cells for this experiment. First, we separated these cells into EMF-group (experimental group) and Control group. The EMF group is placed in the Wave Pro Chamber and exposed under 2.4GHz electromagnetic wave for 4, 8, 16 and 20 hours, while the control is also placed in the Chamber but not in the path of the wave. The reason why we put both groups together in the chamber is because we want to make sure all the conditions are the same, in terms of temperature and humidity. Results Retinal pigment epithelium cell line (graphs will be displayed on my poster and report) 1. SOD, CAT, GPx and GST four antioxidant enzyme activities all decrease. However, there is an increase in GR activity. Retinal Ganglion Cell line 1. SOD, GPx and GST activities all decrease after the EMF exposure. 2. CAT level increases. 3. GR stays the same. Conclusions/Discussion We can conclude that 2.4 GHz EMF exposure does cause more oxidative stress. Epithelium cells are less protective against oxidative stress than the RGC because the enzyme activity in the EMF groups of epithelium cells changes up and down more frequently. SOD, CAT and GST are all compensatory induced for a secondary defense system. In RGC cells, SOD、GPx and GST as the primary barrier (depleted); and induced CAT level for the secondary barrier . GR has no significant influence by the oxidative stress resulting from EMF radiation in our studies. I suggest that the RPE cells is more sensitive as the RGC cells in retina, and therefore the antioxidant#SOD serves as a barrier to protect the body by evidence of depletion; if further oxidative stress continued to occur, the induced CAT and GST can help soothe and protect the retina.

Competition history

• CSEF 2011 Biochemistry/ Molecular Biology · Entry S0517

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