Antioxidant Enzyme Activities of Drosophila melanogaster under the Exposure of WiFi 2.4 GHz Electromagnetic Field

Overview

Objectives/Goals Whether 2.4GHz Electromagnetic Field (EMF) will have an impact on Drosophila melanogaster's antioxidant enzymes. Methods/Materials 1. Collect 3000 randomly selected Drosophila (of the same age) and feed them dry yeast [dry yeast expands the female fruit flies# ovary capacity for more eggs] 24 hours before experiment. 1.Remove the juice plate with eggs on it and replace it with a new juice plate and start the timer for two hours (to synchronize the eggs). 2.Remove the juice plate with eggs on it after two hours (the newly collected one) and place it on the EMF stage in the Wave-Pro Chamber; turn the EMF signal on through the computer for 16 hours. 1.Collect samples at 4hour, 8hour, and 16hour mark respectively. 2.Store samples at -80 degree refrigerator for later antioxidant activities# tests. 3. Repeat above steps for the control group (sham exposure) Drosophila adults collection For the Adults, select 90 fruit flies (not sorted by sex) and put them in a vial with a cotton over it for air. Repeat steps until there are eight sets of vials 4, 8, 16 hours# EMF and control groups respectively. 1. tape the vials onto a board and tape that onto the EMF antenna for exposure. 2. Take out vials that have been exposed the amount of exposure needed. 3. Place 30 fruit flies in one eppendorf to homogenize. Enzyme activities protocol not included on this abstract due to space. Results Embryo - decreased SOD, GPx levels, increased CAT levels, and GR has no significant difference. Adult - decreased SOD levels, and increased CAT, GPx. GR has no significant changes Conclusions/Discussion Drosophila#s embryonic stages exhibit protections against oxidative stress from EMF radiation via decreased SOD and GPx as the primary barrier (depleted); and induced CAT level for the secondary barrier . GR has no significant influence by the oxidative stress resulting from EMF radiation in my studies. However, at the adult stage, only SOD is depleted at the primary defense; CAT and GPx are both induced for the secondary defense system. GR also has no significant changes in our studies. I suggest that the adult stage is not as sensitive as the embryonic stage in Drosophila melanogaster ,and therefore the antioxidant#SOD serves as a barrier to protect the body by evidence of depletion.

Competition history

• CSEF 2008 Biochemistry/ Molecular Biology · Entry S0408

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