The Study of the Effects of Electromagnetic Fields at Various Frequencies upon Cancerous and Noncancerous Cells

Overview

Objectives/Goals The objective of this experiment was to see what effects various frequencies of Electromagnetic fields had on the reproductive growth of cancerous and noncancerous cells. Methods/Materials 1. Culture the cell lines CCL-107, CCL-219, and CRL-2535 according to ATCC procedures. 2. Construct nine electromagnetic field emitting boxes. 3. Take your petri dishes place 5 mL of the growth medium with the cells you want to test in each petri dish. Para film the edges to insure no leaks. Place 4 petri dishes in each of the electromagnetic field boxes and 4 controls in the incubator (do this separately for each cell line). 4. After 48 hours remove the petri dishes from the aluminum boxes in the incubator, take a 500 microliter sample from each petri dish, place those samples individually in different capsules with a 1:1 volume of Trypan blue staining dye. 5. Then pipette 10 microliters and place it in the groves of a hemocytometer and place a glass slide over it and count the number of cells in each of the four 1 square mm corners and divide to get the average. 6. Then multiple by 10 to the 5th power to get the approximate cells in the 5 mL petri dish. 7. Lastly Run a one way ANOVA test and analyze your data. Results # The data I collected regarding electromagnetic field exposure upon Glioblastoma Multiforme (GBM) suggests: 1. The P-value, which is 1.06E-16, provides the evidence to reject the null hypothesis which states that EMFs do not affect the growth of GBM. # The data I collected regarding electromagnetic field exposure upon Leukemia suggests: 1. The P-value, which is .397, leads to the acceptance of the null hypothesis which states that EMFs do not affect the growth of Leukemia. # The data I collected regarding electromagnetic field exposure upon healthy glial cells suggests: 1. The P-value, which is .015, provides the evidence to reject the null hypothesis which states that EMFs affect healthy glial cells in a harmful manner. Conclusions/Discussion Through all the information obtained throughout the course of this experiment I have proven half of my first hypothesis to be correct. GBM when exposed to the various EMF frequencies decreased in cellular reproduction on average by 48.03% amongst all the trials, with P-values supporting these results.

Awards (1)

• Honorable Mention

Competition history

• CSEF 2013 Pharmacology/ Toxicology · Entry S1725

Resources

• CSEF project summary ↗

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