Antibiotic Resistance in Selected Cell Lines

Overview

Objectives/Goals The Objective of this experiment was to determine the resistance of two strains of Chinese Hamster Ovary cells to the antibiotic Geneticin. My hypothesis was that the strain called CHO-M1 would be more resistant to geneticin because this strain had been treated to make it resistant to Geneticin. Methods/Materials Three plates of each strain of CHO cells were grown in each of eight dilutions of Geneticin. These plates were fixed with methanol and stained with Crystal Violet. Next the number of colonies with more than 50 cells were counted and from these counts a cloning efficiency was derived. Results The results of this experiment were that CHO-K1 cells could not survive in concentrations of Geneticin above 500 mcg/ml. However CHO-K1 cells could survive at concentrations of Geneticin up to 2000 mcg/ml, but at much lower cloning efficiencies. Conclusions/Discussion My hypothesis was supported, CHO-M1 cells did have a higher cloning efficiency than that of CHO-K1 cells after exposure to Geneticin. In fact the Geneticin was virtually useless against the CHO-M1 cells. Therefor my conclusion is that microbes that are resistant to antibiotics are a important health hazard because they are difficult to treat.

Competition history

• CSEF 2006 Pharmacology/ Toxicology · Entry J1414

Resources

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