Danger: Antibacterial Resistant Bacteria on the Loose!

Overview

Objectives/Goals To produce antibacterial or antibiotic resistant bacteria. And to count the number of 'generations' that this takes. Methods/Materials Firstly, I swabbed the back of my mouth/throat with a sterile swab, to gather a sample, which I then transferred to a blood agar plate. Then I placed a disk soaked in Amoxicillin in the center of the plate. Then I sealed the plate and placed it in the incubator. Next I repeated this process but soaked the disk in antibacterial soap. After 48 hours, the bacteria had grown and there was a ring that was bacteria free around the disk. Then I took a sterile swab soaked in distilled water and transferred the bacteria on the outskirts of the ring to a new plate, sealed it and put it in the incubator. I repeated this process until there was no longer a zone of inhibition or the antibiotic killed the bacteria. Results The amoxicillin killed the bacteria. However the bacteria on the plate with the antibacterial soap became resistant after three 'generations'. The other plate with antibacterial soap bacame resistant after six 'generations'. Conclusions/Discussion This experiment i have proven that the number of 'genrations' varies in which it takes to produce resistant bacteria. Because it is possible to produce resistant bacteria it is very important that antibiotivc and antibacterial soap be used correctly by society.

Competition history

• CSEF 2002 Microbiology · Entry S1324

Resources

• CSEF project summary ↗

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