Transfecting Zebrafish Scales with Plasmids Using Magnetofection

Overview

I experimented with zebrafish scales because they are a very powerful tool for research. I transfected scales with plasmids or engineered loops of DNA to see whether the cells could express the encoded fluorescent proteins. Transfection is the process by which DNA or RNA is artificially introduced into cells. I conducted my first experiment to determine whether magnetofection, a transfection method that uses a magnet, actually transfects cells within zebrafish scales. This succeeded, after which I used different plasmids, varied the time the scale was over the magnet, and even transfected scales with two plasmids each. This project is very important because it could have many real-world applications, such as helping with osteoarthritis research and providing a medium to cure breast cancer.

Awards (1)

• Selected for CWSF 2026

Competition history

• CWSF 2026 Curiosity & Ingenuity

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