The Salt to my Pepper: The Effects of Utilizing the Bioactive Descriptors of Piperoyl Piperidine (Piperine) on B16-F10 as a Neoteric Treatment for Melanoma

Overview

Melanoma occurs from melanocytes, which produces the pigment cells. Piperine with the application as an ingredient in black pepper, is used in traditional medicine, justified by studies on its biological effects as antioxidant, antitumor, and drug availability-enhancing characteristics potential. The purpose of this study was to examine inhibitory effects of piperine on B16-F10 cell growth to view the ability to decrease cell proliferation. It was hypothesized that piperine would inhibit B16-F10 growth, and higher concentration would result in higher tumor inhibition. T-1 delineated 0.1 uM of piperine, T-2 delineated 0.2 uM, and T-3 delineated 0.3 uM. After 24 hours, the control had an increase in tumor volume by 1025%, T-3, with the most concentration, demonstrated a TGI of 70.23%. T-2 undergoing a 60.96% TGI and T-1 undergoing a 41.63% TGI. Cell proliferation levels dropped dramatically, no matter the cell density, killing B16-F10 growth. Future research includes testing greater amounts of piperine, testing cancers with similar cell function, such as basal and squamous cell, and tests on human cells. Understanding piperine having an inhibitory effect on melanoma, we can accumulate extensive knowledge about the processes involved with cancer onset and growth. From there, we are one step closer to a cure.