The Functional Requirement of Aquaporin1a1 in Zebrafish Vascular Development
ISEF · 2018 Cellular and Molecular Biology Fourth Award
Overview
Aquaporin1a1 is an integral membrane protein that facilitates the transportation of water molecules across a cell. Previous research showed that morpholino knockdowns showed reduced transfer rates of water, which might impact vessel lumenization (Talbot et al. 2015). Therefore, the aquaporin1a1 (aqp1a1) gene is potentially responsible for the regulation of endothelial tube formation and vascular development of zebrafish. CRISPR-Cas9 and a homology-based vector were used to generate precise integration mutants of reporter genes to help visualize the target gene mutation with amplified expression of Red Fluorescent Protein. The mutants showed vascular phenotypes in comparison to the controls and the genetic integration was confirmed to be precise by DNA sequencing, suggesting that aqp1a1 might be required for zebrafish vascular development. Further breeding and statistical analysis would be conducted to strengthen this claim.
Awards (1)
- Fourth Award of $500 $500
Competition history
- ISEF 2018
Resources
Related projects
ISEF · 2025
Investigating Impacts of the E736D COL22A1 Mutation Upon Vascular Stability and Hemorrhagic Incidence in an Embryonic Zebrafish Model
ISEF · 2026
Identification of a Novel TBX2-Dependent Mechanism Linking Pericyte Dynamics to Abnormal Vasculature in Lung Adenocarcinoma
ISEF · 2017
Understanding the Proteins Involved in Regenerative Angiogenesis
ISEF · 2018
Examining the Effect of HIF and Notch in Endothelial Cell Migration and Sprouting
Closest projects by meaning, across every fair and year in the corpus.
Source: Regeneron International Science and Engineering Fair