Potential Neuronal Toxicity Associated with Cellular Exposure to Electronic Cigarette Fluids

Overview

Electronic cigarettes are battery operated devices using nicotine and liquids to produce vapor. They are comprised of three parts: a cartridge, a heating device, and a power source. The cartridge holds e-liquid, nicotine, flavoring, water, and either propylene glycol or vegetable glycerine. When on, the e-cigarette vaporizes the contents in the cartridge. The cells were grown and established before testing began. The e-liquid was diluted using 1 mL of distilled water and 35 micro liters of e-liquid. Four mL of complete growth medium was placed in each of 5 chambers. 1 mL cell culture was placed in each of the 5 chambers. Fifty micro liters of e-liquid solution was placed in each of the 5 chambers. This process was repeated for each strength of e-liquid. The control group followed the same process except no e-liquid solution was added. The test groups were incubated at 37 degrees C for 4 days. The cells were counted using a hemocytometer. This process was completed for each test. The data collected shows that e-cigarette liquid had a negative impact on the cells. The concentration of nicotine did not have as big of an impact as the chemicals in the e-liquid. The e-liquid caused a greater negative impact on the cells than the nicotine. There was a 30.8% reduction of cells from the 0% nicotine e-liquid to the 10% nicotine e-liquid. The nicotine only contributed to 12.44 cell decrease.

Competition history

• ISEF 2017 Biomedical and Health Sciences · Entry BMED005

Resources

• ISEF abstract page ↗

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