NEW ENGLAND NORTHERN Nanovesicles in Broccoli Sprouts as a Naturally Potential Medicine for the Targeted Treatment of Inflammatory Bowel Disease

Overview

Inflammatory bowel disease (IBD) is an incurable small intestine and colon disorder. While exerting anti- inflammatory effects, bioactives in broccoli sprouts cannot treat IBD well probably due to their instability in the GI tract and/or inadequate transit into colon cells. The study aimed to investigate if broccoli sprout-derived exosome (BSDExo) nanovesicles can protect bioactives from the upper GI extreme environments and confer selectively targeted delivery of bioactives to inflamed colon cells in IBD. Nanosized BSDExo with 40.1±17.2 nm encapsulated and protected bioactive sulforaphane for 2 hours in stomach-mimicking acid and 24 hours in intestine-mimicking conditions. 25 ng/mL BSDExo quantified by total proteins promoted the proliferation of normal colon epithelial CCD841 CoN cells with a cell viability of 154±5% (p<0.05). Both CCD841 CoN and Caco-2 cellular uptake of fluorescence-labeled BSDExo significantly increased with more severe inflammation stimulations. Interleukin 8 (IL -8) secretion from inflammation-stimulated normal colon cells was significantly reduced by the BSDExo treatment (p<0.05). BSDExo also significantly recovered the decreased transepithelial electrical resistance (TEER) values caused by inflammations in Caco-2 cells (p<0.05). Overall, the isolated BSDExo improved the stability, inflammation-targeting, and therapeutic efficacy of bioactives in colon epithelial cells via a naturally formed nanostructure. Demonstrating that BSDExo interacts with the targeting gut inflammatory cells and regulates anti-inflammatory responses would be a significant step forward in treating IBD.

Competition history

• JSHS 2023 Category not listed

Resources

• National JSHS abstract book ↗

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