New England Northern Blueberry-Born Nanovesicles as Potential Antioxidants for Treating Neurodegenerative Diseases

Overview

Blueberries, recognized as "super-healthy" fruits, offer benefits like reducing oxidative stress and enhancing cognitive function, potentially protecting against neurodegenerative diseases. However, their high -value bioactives like polyphenolics face challenges in stability and brain delivery. This study explores blueberry- derived exosomes (BBDExo) as nanovesicles that might encapsulate these bioactives, offering improved stability and enhanced cellular uptake, thereby potentially increasing their effective ness in neurological applications. The isolated exosome size was 82.7±6.4 nm and appeared as individual sphere -shaped morphology under scanning electron microscopy (SEM) analysis. 25 µg/mL exosomes quantified by total proteins promoted the best proliferati on of brain endothelial bEND.3 and neuronal SH -SY5Y cells. Brain endothelial bEND.3 cellular uptake of fluorescence -labeled BBDExo significantly. Interleukin 8 (IL -8) secretion from inflammation -stimulated normal colon cells was significantly reduced by th e BBDExo treatment (p<0.05). Overall, the isolated BBDExo improved the stability, brain delivery, and therapeutic efficacy of bioactives in brain cells via a naturally formed nanostructure. Demonstrating that BBDExo interacts with the targeting brain inflammatory cells and regulates anti-inflammatory responses would be a significant step forward in treating neurodegenerative diseases. The characterized exosomes with biomolecules may deliver therapeutic molecules in the brain and target neural cells, leading to improving efficacy in the treatment of neurodegenerative diseases.

Competition history

• JSHS 2024 Category not listed

Resources

• National JSHS abstract book ↗

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