Multifunctional Nanoparticle-Mediated Delivery of miR-200c Restricts the Metastatic Ability of TNBC Cells

Overview

The ability of microRNAs to regulate oncogenes through RNA interference makes them a promising target in cancer therapy. Specifically, miR-200c has gained attention in triple negative breast cancer treatment due to its role in suppressing metastasis, cancer stemness and drug resistance. However, the clinical application of miRNAs is limited by the lack of effective delivery methods. Here, the ECO carrier was optimized to facilitate the cytosolic release of miR-200c. Transfection was evaluated by analyzing respective mRNA and protein expressions of metastasis marker ZEB1 in TNBC cell lines MDA-MB-231 and Hs578T. The ECO/miR-200c nanoparticle downregulated ZEB1 mRNA and protein levels. Additionally, overexpression of miR-200c in MDA-MB-231 cells reduced cancer stemness and cell survival as shown through mRNA expressions of cancer stemness marker BMI1 and cell survival proteins Cyclin D2 and Survivin. Decreased protein levels of drug transporter protein ABCG2 were also observed, implicating miR-200c’s role in drug resistance. In addition, there was decreased migration of miR-200c treated cells compared to the negative control. The data not only demonstrates that miR-200c can suppress metastasis, but it can also decrease the ability for TNBC cells to survive. Looking forward, the effects of miR-200c will be tested in vivo to better evaluate treatment efficacy.

Competition history

• AJAS 2018 Category not listed

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