Multi-Color Magneto-Fluorescent Nanoarchitectures for the Targeted Identification of Exosomes of Triple Negative Breast Cancer

Overview

Due to rising mortality caused by breast cancer, it is necessary to develop early diagnostic techniques for the rapid identification of multiple exosomes of cancer prior to metastasis. Triple negative breast cancer (TNBC) makes up 10 -15% of breast cancers and is characterized by its lack of estrogen, progesterone, and HER-2 receptors. This lack of receptors leads to an absence of targeted therapeutics and higher mortality rates, underscoring the need for early detection for improved patient outcomes. Exosom es are extracellular vesicles found abundantly in the blood, containing biomarkers specific to their cell of origin. If the presence of specific exosomes of TNBC in the blood could be detected, then that would enable early further insight into cancer progression. In this study, we report the synthesis of magneto -fluorescent carbon dot nanoarchitectures for the identification of the Tenascin C, Amphiregulin, and Programmed Cell Death Ligand-1exosomes of TNBC. The nanoarchitectures produced in this study would enable multi-exosome detection, a new avenue that could enable better insight into the behavior of the cancer, prediction of most effective treatment options, and insight in to staging of the cancer. In this study, yellow, green, and blue carbon dots were prepared using orange peel, yellow melon, and cantaloupe organic material. The three carbon dots were coupled with amine functionalized Fe2O3 magnetic nanoparticles (MNPs) us ing the EDC/NHS coupling method. The MNP -carbon dot composites were conjugated with aptamers to form magneto -fluorescent nanoprobes with high quantum yields. The nanoarchitectures were then employed in mixed exosome environments, displaying good specificity enabling multi-exosome detection.

Competition history

JSHS 2025 Category not listed

Resources

National JSHS abstract book

Related projects

ISEF · 2025