Early Detection of Triple Negative Breast Cancer using Multi -Functional Magneto -Fluorescent Carbon Dots

Overview

To combat rising mortality due to cancer, it is necessary to develop early diagnostic techniques for the rapid identification of cancer before metastasis. Triple negative breast cancer (TNBC) makes up 10-15% of breast cancers and is estrogen receptor -negative, progesterone receptor -negative, and HER -2-growth-receptor negative. This lack of receptors leads to late detection of TNBC, resulting in higher death rates. Exosomes are extracellular vesicles found abundantly in the blood, containing biomarkers specific to the cell they originated from. If the presence of exosomes of TNBC could be detected in the blood, then TNBC could be detected early, thus reducing cancer mortality. This study reports the design of magneto -fluorescence carbon dot nanoarchitectures for the identification of the CD -9 exosome of Triple Negative Breast cancer. Red, yellow, and blue carbon dots were prepared using the hydrothermal method. The three carbon dots showed good fluorescence (quantum yield > 8%) and were combined with amine functionalized Fe 2O3 magnetic nanoparticles (MNPs) using the EDC/NHS coupling method. The carbon dots (CDs) and Fe 2O3 MNPs were characterized with various microscopic and spectrosco pic techniques, including TEM, SEM, DLS, FTIR, XPS, EDX and zeta potential measurement. The MNP-carbon dot composites were conjugated with the CD-9 aptamer to form magneto-fluorescent nanoprobes. The nanoarchitectures were employed in exosome solution and displayed good selectivity, highlighting their application as a low-cost, less invasive test to detect TNBC early.

Competition history

• JSHS 2024 Category not listed

Resources

• National JSHS abstract book ↗

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