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Manganese Sulfate Supplemented Caenorhabditis elegans Display Increased Resistance to Oxidative Stress

CSEF · 2006 Pharmacology/ Toxicology

Michael S. Berriman · Villa Park High School, Villa Park

Overview

Objectives/Goals To observe the effects of Manganese Sulfate (MnSO(4)) upon C. Elegans roundworms under Heat Stress, temperature set at 35 Degrees C. Methods/Materials Place 20 Gravid worms of desired strain(s) on an agar plate, 1 plate per strain, with a platinum wire pick. Place the plates in the 35 degree incubator for 2 hours, check deaths under microscope and document. Repeat process, but every 1 hour from then on. Results The variable data set of worms, which was the MnSO(4) supplemented population, showed increased longevity under the circumstance of increased temperature. Conclusions/Discussion Worms treated with MnSO(4) displayed increased thermotolerance, as they were better able to internally neutralize ROS.

Summary statement

I treated C. elegans with MnSO4 in order to reduce Oxidative stress, and the Heat Stress Assay performed helped to display that MnSO4 does increase the worms' lifespan.

Help received

Used lab equipment at Cal State Fullerton under Dr. Srinivasan ; Father edited report; Jessica Hessom of CSUF Biochemistry Department helped with protocol and poster

Competition history

  • CSEF 2006 Pharmacology/ Toxicology · Entry S1405

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