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Leukemia Chemotherapy

CSEF · 2009 Pharmacology (Senior Division Only)

Natalie A. Chen · Upland High School, Upland, San Bernardino County

Overview

Objectives/Goals My objective was to determine which drug combination [Clofarabine and Busulfan vs. Clofarabine and Melphalan] is more synergistic in DNA damage on Acute Lymphoblastic Leukemia [ALL] cells. I hypothesize that Clofarabine in combinations with Busulfan will have better synergistic results. Methods/Materials Methods: Western Blotting, MTS assay, Calcusyn program and cell culturing. Materials: Reh [human leukemia cell line], general pre-clinical laboratory equipment. Results The Calcusyn program showed Clofarabine in combination with Busulfan to have better synergistic results. The Western Blotting tracing the yH2AX protein expression also showed Clofarabine in combination with Busulfan to have better synergistic results. Conclusions/Discussion The hypothesis was correct, Clofarabine in combination with Busulfan does have better synergistic effect than Clofarabine in combination with Melphalan.

Summary statement

Pre-Clinical study comparing Clofarabine in combination with Busulfan versus Melphalan on Reh.

Help received

City of Hope Eugene and Ruth Roberts Summer Academy Program. Mentors: Dr. Shika and Dr. Gaur.

Competition history

  • CSEF 2009 Pharmacology (Senior Division Only) · Entry S1807

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