Killer Karts

CSEF · 2006 Pharmacology/ Toxicology

Overview

Objectives/Goals My objective is to see whether wiping down grocery cart handles reduces bacterial growth and which of 3 different disinfectant wipes prevents the most bacterial growth. Methods/Materials For each of 3 different disinfectant wipes, 3 wipes were used to wipe down 9 randomly selected grocery cart handles. The cart handles were swabbed with a sterile swab after drying for 30 seconds and cultured on sterile agar plates. An additional 3 cart handles were swabbed without being wiped by any disinfectant wipe and cultured on agar plates. Three agar plates were also cultured with a sterile swab and used as controls. The plates were then incubated for a total of 96 hours with the number and appearance of bacterial colonies recorded for each plate. The experiment was repeated 2 more times for a total of 3 trials. The growth of bacteria was compared by totaling up the number of colonies found for each type of wipe, nondisinfected and control plates. Results The control plates had 0 bacterial colonies at 96 hours. The non disinfectant plates had a total of 377 colonies at 96 hours. The SaniCart Wipes plates had a total of 6 colonies at 96 hours. The Wet Ones Antibacterial Towelettes plates had a total of 6 colonies at 96 hours. The Clorox Disinfectant Wipes plates had a total of 3 colonies at 96 hours. Conclusions/Discussion My conclusion is that the disinfectant wipes significantly reduced the number of bacterial colonies growing. I also conclude that the Clorox Disinfectant Wipes were slightly more effective at reducing the growth of bacteria than either the SaniCart Wipes or the Wet Ones Antibacterial Towelettes.

Summary statement

Which of three wipes disinfects grocery cart handles best?

Help received

My mom helped me type my report and assisted me in my experiments. My dad helped me graph my data, take pictures of the plates and helped with my display

Competition history

  • CSEF 2006 Pharmacology/ Toxicology · Entry J1427

Resources

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