Effects of Pesticides on Mitochondrial Activity and DNA Levels in Mouse Myoblast Cells

Overview

Objectives/Goals The focus of this project was to determine whether pesticides permethrin, carbaryl, and imdacloprid, affected mitochondrial activity and mitochondrial DNA levels in C2C12 mouse myoblast cells. Methods/Materials Cells were treated with dosages of pesticide with similar concentration to the LD50 and LD25 toxicity in rats for 24 hours. The mitochondrial activity was measured as the basal and maximal oxygen consumption rates (OCR) in the mitochondrial stress test. To determine the amount of mitochondrial DNA, the ratios of mitochondrial DNA to nuclear DNA were determined from treated samples by amplification using live Polymerase Chain Reaction. Results The LD50 permethrin treatment significantly reduced the basal OCR (p = 0. 04) but not the maximal OCR. The LD25 permethrin treatment and carbaryl and imidacloprid treatments did not have significant effects on basal and maximal OCR. All the pesticide treatments did not affect mitochondrial DNA levels except for the LD50 imidacloprid treatment, which significantly affected the mitochondrial DNA levels (p = 0.05). Conclusions/Discussion Permethrin and carbaryl affected the mitochondria#s ability to make ATP not by lowering amount of viable mitochondrial DNA, but by affecting the electron transport chain.

Competition history

• CSEF 2014 Pharmacology/ Toxicology · Entry S1718

Resources

• CSEF project summary ↗

Related projects

Closest projects by meaning, across every fair and year in the corpus.

Browse more like this

• All Biomedical and Health Sciences projects
• CSEF Biomedical and Health Sciences projects
• Every CSEF 2014 project