Digestion Rate of Starch Solution in Amylase Solution at Various Temperatures and Concentrations

Overview

Objectives/Goals THE GOAL OF THIS PROJECT IS TO INVESTIGATE AT WHICH TEMPERATURE AND CONCENTRATION WOULD AMYLASE ENZYME SOLUTION HAVE THE GREATEST DIGESTION RATE ON STARCH SOLUTION. Methods/Materials AMYLASE ENZYME SOLUTIONS (1%, 5% & 10%); STARCH SOLUTION (2%); WATER BATHS (ICE COLD, BODY TEMPERATURE, FEVER TEMPERATURE, BOILING WATER); BUFFER SOLUTION; TEST TUBES & RACK; IODINE INDICATOR SOLUTION; HEATING ELEMENT; 10-HOLE SPOT PLATES; DIGITAL TIMER; THERMOMETER Results THE RESULTS OF THE EXPERIMENT DID NOT SUPPORT MY HYPOTHESES. THE RESULTS INDICATED THAT BODY TEMPERATURE HAS THE GREATEST DIGESTION RATE WITH THE ICE COLD BATH TEMPERATURE HAS THE SECOND GREATEST DIGESTION RATE. THE RESULTS ALSO INDICATED THAT AMYLASE ENZYME AT 10% CONCENTRATION AND BODY TEMPERATURE HAS THE GREATEST DIGESTION RATE WITH THE ICE BATH AT THE SAME CONCENTRATION HAS THE SECOND GREATEST DIGESTION RATE. Conclusions/Discussion MY HYPOTHESES WERE NOT SUPPORTED BY THE TEST RESULTS. RESEARCH INDICATED THAT ADDING HEAT TO SOME ENZYMES MAKES THEM MORE EFFECTIVE HOWEVER WITH AMYLASE THE RESULTS INDICATED THAT AMYLASE IS MOST EFFECTIVE AT BODY TEMPERATURE AND ADDING HEAT MAKES THE ENZYME LESS EFFECTIVE. THE RESULTS ALSO SUGGEST THAT REDUCING THE TEMPERATURE DOES NOT MAKE THE ENZYME LESS EFFECTIVE.

Competition history

• CSEF 2014 Biochemistry/ Molecular Biology · Entry J0503

Resources

• CSEF project summary ↗

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