Characterization of Engineered Chimeric Protein LLHP Repression Through the Induction of DNA Looping

Overview

DNA looping is a method of gene regulation that plays an important role in controlling transcription. Previous research has shown that DNA looping occurs as a method of gene regulation when transcription factors that are bound to operators interact and tetramerize, causing the DNA to form a loop. Another area that has been explored is how intersite spacing affects DNA’s ability to loop. As the operator spacing changes, the helical shape of DNA affects the proteins bound onto the operator's ability to interact and form a loop. Although it is known that the transcription factor's ability to tetramerize correlates to DNA looping, it has not been explored how 49 the addition of a tetramerization domain to another protein could cause DNA looping. It is my hypothesis that by adding a tetramerization domain to a protein construct that does not induce DNA looping usually, will be necessary and sufficient to induce looping in said protein. I will explore this using repeat ONPG assays to test the level of repression which should increase and decrease depending on whether LLhP is inducing looping in DNA. By exploring how protein domains work independently of each other, it allows for a greater understanding of how to engineer proteins for the desired function. KENTUCKY

Competition history

• JSHS 2023 Category not listed

Resources

• National JSHS abstract book ↗

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