Bioassay of Garlic Extracts on Root-knot Nematodes to Determine the LC50

Overview

Objectives/Goals My objective was to determine the LC50 of garlic extracts on root-knot nematodes exposed for twenty-four hours. I believe the LC50 of garlic extract will be 10-g/l, because from my previous experiment 20-g/l had 100% mortality. Methods/Materials I extracted root-knot nematodes from 1 liter of soil by sieving the soil and putting it into a mist chamber. I made my garlic extract by cutting up 20 grams of garlic and putting it into a liter of water. I exposed the root-knot nematodes to 20-g/l, 10-g/l, 5-g/l, and untreated water. I checked the mortality after 24 and 48 hours. Results The 20 and the 10-g/l both had 100% mortality. The 5-g/l had 40.3% mortality and the untreated water had 0% mortality. I used log probability paper to estimate the LC50 to be 6.5-g/l. Conclusions/Discussion My conclusion is 6.5-g/l is the approximate LC50 of garlic extract on root-knot nematodes. I need to do more experiments narrowing the concentrations closer to the 6.5 range.

Competition history

• CSEF 2002 Pharmacology/ Toxicology · Entry J1416

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