Antibiotic Resistance: Synthetic vs. Natural

Overview

Objectives/Goals I am testing which type of antibiotic, natural or synthetic, leaves a larger zone of inhibition against bacteria. Methods/Materials I used LB agar plates, and s. epidermidis bacteria. the antibiotics that I used were (natural) ACV (raw apple cider vinegar), honey, garlic, (synthetic) tetracycline, penicillin, and ampicillin. I inoculated six antibiotics into each agar plate and let it grow for two days in and incubator at 38 degrees Celsius. After two days, I measured the zones of inhibition in millimeters. Results Natural antibiotics showed more consistency, while the synthetic ones only worked well in the beginning, but had little to no zone of inhibition in trials 2 and 3. The mean for pharmaceutical was 9.19 mm. The mean for natural was 10.62 mm. Garlic had the largest (average) zone for natural, and ampicillin had the largest (average) zone for synthetic. Conclusions/Discussion Synthetic antibiotics were not as consistant as the natural. Natural antibiotics, on average, left a larger zone of inhibition than synthetic antibiotics.

Competition history

• CSEF 2017 Microbiology (Medical) (Junior Division Only) · Entry J1701

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