A Novel Cancer Killing Strategy: Direct Protein Delivery of Caspase-3
CSEF · 2002 Pharmacology/ Toxicology
Overview
Objectives/Goals The present project was undertaken to test the feasibility of a novel strategy- direct delivery of functionally active proteins to kill tumor cells. The commercially available protein delivery reagents Profect P-1 and Profect P-2 (Targeting Systems, Ca) were used in delivering Alexa-488 conjugated Histone H-1, b galactosidase and activated caspase-3. Caspases are enzymes, which in their active form can induce cell death by a mechanism termed as apoptosis- a fundamental biochemical pathway for normal tissue homeostasis, cellular differentiation, and development within a multi-cellular organism (Simizu, 1998). The direct delivery of Alexa-488 conjugated Histone H-1 was delivered using Profect P-2. A bright yellow color was observed under fluorescent light. The yellow is a nuclear localization signal activated once the protein had efficiently entered the cells nucleus. The delivery of b galactosidase was confirmed by a blue color, which stained the nucleus once the protein had entered the cells nucleus. Once the efficient delivery of Alexa-488 conjugated Histone H-1 and b galactosidase was successfully achieved the delivery of activated caspase-3 into 80% confluent cells was acheived using Profect P-2 (Targeting Systems, Ca).
Summary statement
Using protein delivery reagents in order to introduce active caspase-3 into an MCF-7 cell line, in order to kill the targeted cells in the form of apoptosis.
Help received
Dr. R. Walia at Targeting Systems, Ca
Competition history
- CSEF 2002
Resources
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