A Novel Analysis of Cosmetic Methylparaben in Artificial Amniotic Fluid

Overview

This study explored the effects of varying concentrations of cosmetic methylparaben on the pH, viscosity, and glucose levels of artificial amniotic fluid. It was hypothesized that increasing methylparaben concentrations in the amniotic fluid would lower pH , increase viscosity, and elevate glucose levels. Solutions containing 0 ng/mL, 8.02 ng/mL, and 16.04 ng/mL methylparaben were prepared, with five replicates for each concentration. pH levels were measured using a calibrated Vernier pH glass -body sensor, r evealing a slight yet statistically significant decrease in average pH across the concentrations. While the results supported the hypothesis that methylparaben decreases pH, there was a non -linear trend observed that warrants further investigation. Viscosity was calculated using the falling-sphere method, where a sphere of known density was dropped into the solutions to find the velocity, which was then put into the viscosity equation. Methylparaben showed no significant effect on viscosity; however, the method used may have limitations in reliability. Finally, glucose levels were assessed with test strips designed for water-based solutions, which included a color chart corresponding to glucose concentrations of 0, 36, 90, 270, and 720 mg/L. Average glucose levels remained consistent across all concentrations, indicating no measurable impact of methylparaben. Methylparaben could have an effect on glucose levels, but the minuscule concentrations found in female amniotic fluid during pregnancy may not be high enough to produce any actual impacts.

Competition history

• JSHS 2025 Category not listed

Resources

• National JSHS abstract book ↗

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