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Zap! Analyzing the Effectiveness of Oil Free Acne Cleansers and the Eradication of Escherichia coli

CSEF · 2011 Product Science (Biological) (Junior Division Only)

Tatiana C. Trejo · Sanger Academy Charter School, Sanger, Fresno County

Overview

Objectives/Goals The purpose of my investigation was to find a store bought acne cleanser to help eliminate bacteria and acne. Methods/Materials For my experiment, I used E.coli, Neutrogena Oil-Free Acne Wash, Clean & Clear Oil Free Foaming Facial Cleanser, CVS Oil Free Foaming Facial Cleanser, agar plates, filter paper, test tubes, sterile cotton swabs, distilled water, and chlorine bleach. I had my coach swab E.coli onto two agar plates and I grew them for 3 days. Next, I swabbed the grown E.coli onto 75 different agar plates and then labeled the agar plates with the cleanser's name and trial number. I then mixed water and the cleanser into a test tube and, dipped a filter paper disk into the solution and, place it onto a agar plate with the use of tweezers. I grew the plates again for three days and then I found the width of the killed bacteria by measuring the width between outside edges of the filter paper disk and the circle of killed E.coli. Lastly, I added bleach and water to a bucket and put the agar plates into the bucket and let sit. I disposed of the agar plates into a waste basket. Results The results of my experiment showed that the Neutrogena brand cleanser had an average of 3.232 cm of eliminated bacteria while the Clean & Clear had an average of 2.624 cm of bacteria eradicated. Lastly, the CVS brand cleanser had an overall average of 2.204 cm of killed bacteria. Conclusions/Discussion From my experiment, i learned that the Neutrogena and Clean & Clear brand can be very effective in eradicating E.coli. The CVS brand, though much less expensive is not as effective and is a generic store brand.

Summary statement

I chose to do this experimentation because I wanted to find an effective way to help clear acne.

Help received

Nathan Whittington, a science teacher at the nearby high school, provided the Escherichia coli. Mother and father, helped with the layout and assembly of my board. Carrie Given and Jewely Lopez-Lickey, science teachers, helped me with my procedure.

Competition history

  • CSEF 2011 Product Science (Biological) (Junior Division Only) · Entry J2017

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