Wolbachia vs. Spiroplasma

Overview

Objectives/Goals I did my research project on Wolbachia and Spiroplasma. These two Bacteria infect many insect species are and are passed down from generation to generation through ovaries. My investigative question was; does Wolbachia affect the infection rate of Spiroplasma? Methods/Materials I went to Big Sur and capture fruit flies from several locations. I established 38 inbred lines from individual females, and used polymerase chain reaction (PCR) to see whether each line was infected with Wolbachia or Spiroplasma or both. Results We found Spiroplasma infection = 0. Due to this, my hypothesis couldn't really be tested and my results are inconclusive. My data did show the approximate infection rate for Wolbachia and Spiroplasma when infection > 0. The infection rate for Wolbachia is around 62.5% and Spiroplasma is almost certainly less than 9% but more probably less than 2.5%. Conclusions/Discussion My Hypothesis stated that there would be a change in the rate of Spiroplasma if the flies carried Wolbachia. Unfortunately, my results said my Null, there would be no change, was supported.

Competition history

• CSEF 2010 Microbiology · Entry J1706

Resources

• CSEF project summary ↗

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