Understanding Plant Defense Responses Against Green Peach Aphids (GPA)

AJAS · 2022 Plant Science

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Overview

This project aims to identify interactors of RGPA1, a gene which induces plant defense responses, by using a Yeast two-hybrid screening. Potential interactors are tested to see whether they may provide resistance to aphids, benefitting the agricultural community and providing an alternative to destructive pesticides.

Video

My Story

My research journey officially began in the summer of 2021 in the lab of Dr. Jyoti Shah, Chair of the Department of Biological Sciences at the University of North Texas. My mentor, Dr. Anil Girija, a postdoctoral researcher, introduced me to the process of research and taught me many things I needed to know to complete this project. I dedicated many hours over the year to this project and have not once fell short of being awed by the power of science. Although my experience in a lab was relatively new, my curiosity to explore and uncover mysteries has always persisted since a young age. Being inducted as an AJAS fellow is an honor to be named one among many accomplished young scientists.

Major Assistance

This research was conducted from June 2021 to present at the University of North Texas under the guidance of:

Dr. Anil Girija, Post-Doctoral Researcher, UNT

Dr. Jyoti Shah, Chair/University Distinguished Research Professor, Immunity and stress response in plants, UNT

References:

Addgene: pEZY202. (2021). Addgene.org. https://www.addgene.org/18705/pJG4-5 Sequence and Map. (2016). SnapGene. https://www.snapgene.com/resources/plasmid-files/?set=yeast_plasmids&plasmid=pJG4-5

‌Ho, Chin-Min Kimmy, et al. "Modulators of stomatal lineage signal transduction alter membrane contact sites and reveal specialization among ERECTA kinases." Developmental Cell 38.4 (2016): 345-357.

Matiolli, Cleverson Carlos, and Maeli Melotto. "A comprehensive Arabidopsis yeast two-hybrid library for protein-protein interaction studies: a resource to the plant research community." Molecular plant-microbe interactions 31.9 (2018): 899-902.

Song, Chun-Peng, and David W. Galbraith. "AtSAP18, an orthologue of human SAP18, is involved in the regulation of salt stress and mediates transcriptional repression in Arabidopsis." Plant molecular biology 60.2 (2006): 241-257.

Images (16)

Awards (1)

  • AJAS Fellows Badge

Competition history

  • AJAS 2022 Plant Science

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