Transgene Expression of Wild Type and Mutant Myosin Binding Protein C (MyBP-C) in the Hearts of MyBP-C Knock Out Mice
CSEF · 2003 Biochemistry/ Molecular Biology
Overview
Methods/Materials Familial Hypertrophic Myopathy (FHM) is a disease in which the heart is enlarged to compensate for mutations in muscle proteins. Twenty to thirty percent of cases of FHM were found to have mutations in MyBP-C. To determine the significance of MyBP-C, the MyBP-C gene was "knocked out" through gene targeting and deficits in phenotype were determined. To test specific hypotheses regarding MyBP-C, transgenes encoding for normal and mutant MyBP-C are inserted into the genome of knockout mice. To test the effectiveness of the transgene to restore or modify function, homozygous (knockout gene) mice testing positive for the transgene are tested for the presence of transgene MyBP-C. Results From CTW mice lines 90, 984, and 982, only line 90 has showed strong expression for transgene MyBP-C and none of the CTP mice showed expression. Conclusions/Discussion This low expression rate may be due to low copy numbers of the transgene in the other lines.
Summary statement
To determine whether the phosphorylation site plays a role in the function of MyBP-C in the heart
Help received
Used lab equipment at the University of Wisconsin, Madison under the supervision of Dr. Samantha Harris and Dr. Richard Moss; Participant in the NASA SHARP Plus
Competition history
- CSEF 2003
Resources
Related projects
ISEF · 2016
The Effects of Fetal Troponin T on the Cardiac Remodeling of Hypertrophic Transgenic Hearts
AJAS · 2017
Investigating the Role of Two New Genes Potentially Involved in Lipid Metabolism
CSEF · 2016
Effects on Mouse Growth of Postnatal Novel Protein Kinase Gene Deletion in Endothelial Cells
ISEF · 2023
Investigating the Impact of Pyruvate Kinase Muscle Isoform 2 (PKM2) Knockout on Cardiac Murine Cellular Hypertrophy
ISEF · 2016
Drosophila Mutant Holdup Arises from a Mutation in a Conserved Gene Required for Muscle Integrity
CSEF · 2006
Keratins Gone Bad: A Look at the Causes of a Rare Skin Disease
ISEF · 2016
Determination of Efficacies of Somatic Muscle Cell Transcription Factors for Direct Reprogramming into Induced Muscle Stem Cells
ISEF · 2024
Creation of a Duchenne Muscular Dystrophy Myoblast Cell Culture Model by Knocking Out the Dystrophin Gene Using CRISPR/Cas-9 for High Throughput Drug Screening
Closest projects by meaning, across every fair and year in the corpus.
Browse more like this
Source: California Science & Engineering Fair public projects