Toxic Light Exposure on Yeast

Overview

Objectives/Goals THE OBJECTIVE OF THE PROJECT IS TO FIND OUT IF EXPOSURE TO ULTRA VIOLET LIGHT AFFECT YEAST FERMENTATION RATE AND/OR ALTER PROTEINS IN YEAST, THUS INDICATING THAT LIGHT EXPOSURE CAN BE TOXIC TO CELLS. Methods/Materials THREE TESTS OF 6 JARS OF YEAST AND SUGAR EACH WERE EXPOSED TO UV LIGHTS. EACH TEST HAD 2 CONTROLS PROTECTED FROM EXPOSURE. FERMENTATION RATES WERE OBTAINED WITH A REFRACTOMETER, AND TEMPERATURES WERE RECORDED USING THERMOMETER READINGS. DATA WAS COMPARED TO SEE IF FERMENTATION RATES DIFFERED COMPARED TO CONTROL. PAPER CHROMATOGRAPHY WAS USED TO EXAMINE AND COMPARE PROTEINS IN SAMPLES EXPOSED TO LIGHT VERSUS THE CONTROLS. Results THE SUGAR FERMENTED SLIGHTLY FASTER IN THE EXPOSED SAMPLES OF YEAST OVER TIME. HOWEVER, IN THE BEGINNING OF EXPOSURE, THE CONTROLS WERE FERMENTING FASTER. IN THE PAPER CHROMATOGRAPHY TESTS, THERE WAS A VISIBLE DIFFERENCE IN THE PROTEIN PATTERS BETWEEN THE EXPOSED SAMPLES VS THE CONTROL. Conclusions/Discussion EXPOSURE TO UV LIGHT DID AFFECT YEAST FERMENTATION RATES. THE NON-EXPOSED CONTROLS ENDED UP REACHING ITS FINAL FERMENTATION GRAVITY POINT FASTER THAN THE EXPOSED TEST SAMPLES. THE PROTEIN ALTERATION TESTS DID SHOW A DIFFERENCE IN PROTEINS WHEN EXPOSED TO LIGHT. THE RESULTS DID SHOW A DIFFERENCE CAUSED BY LIGHT EXPOSURE, BUT NOT WHAT TYPE OF PROTEIN CHANGE OCCURRED.

Awards (1)

• Fourth Place

Competition history

• CSEF 2015 Biochemistry/ Molecular Biology · Entry J0515

Resources

• CSEF project summary ↗

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