fairfolio
Saved Projects

The Resistance of Bacteria

ISEF · 2022 Microbiology

Brotherton, Carter · Great Mills High School

Is this your project?

Overview

Disinfectant has been a prominent tool to clean surfaces, and now more than ever it is important for it to be used effectively. The purpose of the experiment was to see if the dilution of a disinfectant has an effect on the resistance of bacteria, in this case a non-pathogenic strain of E. Coli. The experiment consisted of 4 different dilutions of bleach being diffused onto plates cultured with E. Coli. The control concentration had no bleach and was just distilled water, the second had a concentration of 10%, the third had a concentration of 1%, and the fourth had a concentration of 0.1%. Each agar plate was split into 4 sections with a diffusion disk placed in the center of each. Measurements of the inhibition zone around each plate were recorded and then the closest colony from each plate was cultured onto a new plate to regrow. The first round of testing showed 10% with the least resistant bacteria and 0.1% with the most. As the rounds continued into the third day, each plate had bacteria growing closer, showing their increase of resistance. Both the 1% and the 0.1% ended up with less than a .5 mm range. Overall, decreasing the concentration of disinfectant allowed the E. Coli to become more resistant.

Working on something like this? See what's been done and what's still open.

Competition history

Resources

Related projects

Closest projects by meaning, across every fair and year in the corpus.

Browse more like this

Report a problem

Source: Regeneron International Science and Engineering Fair

Save projects to your library

Sign in with Google to keep track of projects you find interesting, organized into folders. An account also raises your daily allowance for “Has this been done?”, and lets you create a key for the MCP server with a much higher limit than anonymous use. Browsing stays public.

Continue with Google