The Effects of Plasma Treatment on the Growth Behavior of Plant Seeds

CSEF · 2011 Plant Biology Third Award

Overview

Objectives/Goals The objective of my science project was to compare the growth characteristics of plasma-treated plant seeds. A plasma is an ionized gas that consists of ions (positive or negative), radicals (excited neutral species), electrons, and radiation (UV/VIS/IR). My hypothesis was that the reactive ion and atom species of a plasma could affect the outer shell of plant seeds, causing enhanced plant-growth characteristics, but this effect would probably depend on seed type and plasma type. Methods/Materials Two types of plant seeds (Bush Bean seeds and Soy Bean seeds) were exposed to three types of atmospheric-pressure plasmas (air, nitrogen/N2, and carbon dioxide/CO2). Each atmospheric-pressure plasma was created by filling a glass-TEE with gas and a high voltage was applied across two sharp metal electrodes to ionize the gas and create an arc discharge. Three plasma exposure times were studied; (1 minute, 5 minutes, and 50 minutes), and seed temperature was kept below 60 C to prevent seed deterioration. Plant height was used to compare growth characteristics of plasma-treated seeds and un-exposed control seeds. Results 1. Bush Bean seeds showed increased growth for all plasma treatments a. 1 minute Air plasma treatment: 90% increased plant height b. 5 minute N2 plasma treatment: 80% increased plant height c. 5 minute CO2 plasma treatment: 40% increased plant height 2. Soy Bean seeds showed reduced growth for all plasma treatments a. 50 minute Air plasma treatment: 35% reduced plant height b. 50 minute N2 plasma treatment: 65% reduced plant height c. 50 minute CO2 plasma treatment: 50% reduced plant height Conclusions/Discussion Plasma treated plant seeds can have increased plant growth vs. un-treated control seeds, but the results depend on seed type, plasma type, and treatment time. The results could have important implications to improve plant growth for farmers.

Summary statement

Plant seeds exposed to an atmospheric-pressure plasma can result in enhanced growth behavior

Help received

Mr. Alan Kramme, VP of Products at Ace Glass Inc. (NJ) for donating the glass Tee, Teflon plugs, and Teflon insulating support rod. Mr. Andrew Del Gatto, owner of DELGA PROTOTYPE, Engineering and Precision Machining for drilling holes in the Teflon plugs for the gas inlet and outlet lines. Father helped

Awards (1)

Competition history

  • CSEF 2011 Plant Biology · Entry S1907

Resources

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