The Effect of UV Light Sterilization on Bacteria Cultures

Overview

The purpose of this project was to see how much Ultraviolet (UV) light exposure is required to kill bacteria cultures. I took 5 different bacteria that can either be found on menus or that can be transferred to the menus. I used E coli- mm294, E coli- DHS5 alpha, Bacillus cereus, Bacillus brevis, and Staphylococcus epidemidis. I would get an inoculating loop and make a line through the test tube with it and then transfer that bacteria with the loop by doing a zig zag motion onto a petri plate. Once I got through all the 188 plates I would set up the UV light. I would set the plates under the UV light for the certain time period that plate was assigned to, either 5 minutes, 10 minutes, 20 minutes, or 30 minutes. Then I would take the plates out and wait for all the plates to be done before I put them in the incubator. Once I put it in the incubator, I set the temperature to 32°C and recorded percent coverage for 5 days. For my control, I had two of them, a positive and a negative. For my positive control I had bacteria on the plate and put them under the UV lights. For my negative I had no bacteria but then put them under the UV lights.

Competition history

• AJAS 2019 Category not listed

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