The Effect of Heteractis magnifica on the Cell Viability of Multicentric Canine Lymphoma

Overview

Objectives/Goals In this study, cytotoxicity induced by Heteractis magnifica venom was investigated using a hemocytometer and a trypan blue solution to determine malignant canine lymphoid CLL-1390 cell viability. Methods/Materials Heteractis magnifica 8 mL of Canine Lymphoma cells Hemocytometer 0.4% solution of trypan blue in buffered isotonic salt solution Suspended 6 mL of CLL-1390 cells on 12 mL of Heteractis magnifica venom to understand its effect on the cell viability. Results The results of the petri-dishes with the addition of Heteractis magnifica venom ranged from 10.16% to 15.5%, a significant decrease from the 79.9% viability rate of the dish in which the venom was not introduced. The first dish (#1) had a 15.5% viability. The second dish (#2) had a 12.8% viability. The third dish (#3) had a 10.16% viability. The average was calculated to be 12.82%. Conclusions/Discussion The overall aim of this study was to determine if Heteractis magnifica venom affects the cell viability of multicentric canine lymphoma. According to the data collected, the hypothesis, if the Heteractis Magnifica venom is introduced to the multicentric canine lymphoma cells, then multicentric canine lymphoma cell viability will be significantly reduced, appears to be supported. As suggested by the evidence, the venom showed a significant reduction of cell viability. Additional studies may confront complications with the expression of Bcl-2 proteins, anti-apoptotic proteins, that challenge therapeutic capabilities and inhibit apoptosis. Fortunately, in this study the cytolytic compounds surpassed the inhibitory protein. Other studies may also confront the dilemma, that the venom is saturated in water. To yield more accurate and error free results in the future an automated cell counter would be used. If the experiment were to be repeated, WST-1 assays would be utilized to determine exact cytotoxicity levels. The next step would likely be to identify the bioactive traits in which the actinoporins possess, and formulate an appropriate therapeutic. Future studies should include the investigation of the venom#s safety, efficiency, and tolerance doses. Future studies should also utilize human cells in replace of canine

Awards (1)

• Fourth Place

Competition history

• CSEF 2017 Microbiology (Medical) (Junior Division Only) · Entry J1703

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