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Spectroscopic Analysis of Crystal Violet in Chymotrypsinogen A-Surfactant Solutions

CSEF · 2006 Biochemistry/ Molecular Biology

Benjamin G. Jimenez · Bravo (Francisco) Medical Magnet High School, Los Angeles

Overview

Objectives/Goals The objective is to investigate the protein form-function relationship in photoresponsive surfactant and Crystal Violet solution after exposure to various light energies in order to observe possible changes in protein conformation. Methods/Materials 10 Solutions were made with azoTAB surfactant concentrations ranging from 0-20 mM. Each solution was ~0.50 mL with 10 mg/mL chymotrypsinogen-A protein and 10 micromolar crystal violet. Solutions were analyzed with UV-Vis Spectroscopy under two conditions of visible light and UV-light. The CV peaks were curve fitted to determine the maximum wavelength and Absorbance at the max wavelength. Results Different light illumination causes a shift in the max wavelength and a change in Absorbance of the solutions. Upon changing Visible to UV light environments, the observed max wavelength decreased and there was an increase in Absorbance. Conclusions/Discussion My conclusion is that the differences in Absorbance and wavelength indicate there is a change in the form of the protein.

Summary statement

My project is about protein misfolding and how the protein form-function relationship may be manipulated through the use of photoresponsive surfactants.

Help received

Used lab equipment at the University of Southern California under the supervision of USC graduate student Andrea Hamill.

Competition history

  • CSEF 2006 Biochemistry/ Molecular Biology · Entry S0406

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