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Single-Molecule Transcriptome: Wide Dynamics of Translation

CSEF · 2019 Biochemistry/ Molecular Biology

Gary J. Song · King (Martin Luther, Jr.) High School, Riverside, Riverside County

Overview

Objectives The goal of this experiment was to conduct a further study into the dynamics of translation using single- molecule FRET techniques. We analyzed mRNA traces to attempt to find a possible correlation with the protein translational efficiency and binding rate of the eIF4E protein. Methods Yeast, Fluorescence dye, RS II, eIF4E protein. Annealed dye onto yeast mRNA then ran traces through the RS II machine. Results Found a 50x differences between the binding rate of difference mRNAs. The graph of our total results was found to be made of many small normal models. Conclusions Our results seem to confirm that there is a possible correlation with translational efficiency. This leads us to believe eIF4E might be the limiting step of Translation, and using this fact will allow us to possible control protein synthesis.

Summary statement

Finding a correlation between eIF4E binding and protein synthesis by looking at the eIF4E binding rates.

Help received

I conducted most of expirement. My graduate student(Burak Cetin) helped supervise the procedure to check for any mistakes and taught me how to analyze the data through MATLAB.

Competition history

  • CSEF 2019 Biochemistry/ Molecular Biology · Entry S0521

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