See C Stay: The Effect of Preservation Technique on the Amount of Vitamin C in Orange Juice

CSEF · 2012 Biochemistry/ Molecular Biology

Overview

Objectives/Goals The objective of this experiment was to learn the effect of preservation technique on the amount of vitamin C in orange juice. Methods/Materials Orange juice samples were preserved in the freezer, the refrigerator, by pasteurization and by dehydration. Freezing/refrigeration required: plastic bags, orange juice, and a freezer/refrigerator. The pasteurization process required: canning jars, orange juice, a pot to boil the orange juice in, and a small graduated cylinder. The dehydration process required: home dehydrator, orange juice and plastic baggies. The iodine titration method was used to determine the amount of vitamin C in orange juice/standard vitamin C solution. This part of the process involved: burete, Erlenmeyer flask, orange juice, iodine solution, starch indicator solution, and the vitamin C solution. Results The frozen samples turned out with the highest concentration of vitamin C with refrigerating in second and dehydration closely behind it. Pasteurization came out with the lowest amount. Conclusions/Discussion Based on my data the first three methods had more Vitamin C than what it started out with and I chalk this up to error on my part. I also conclude that I did not test for a long enough period of time be able to get significant results but that it is probable that freezing would preserve the vitamin C in orange juice the best. But with the data that I got, the numbers are not really different because they were only preserved for a week, not long enough to actually have noticeable change.

Summary statement

My project tests four different home preservation techniques of orange juice to see which one maintains the vitamin C concentration the highest.

Help received

Dad made me a ring stand out of wood for lack of one and helped me squeeze the orange juice in the begining to make the process go faster.

Competition history

  • CSEF 2012 Biochemistry/ Molecular Biology · Entry J0507

Resources

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