Sanitization of Black Tea Using Ultraviolet Radiation
CSEF · 2016 Microbiology (General)
Overview
Objectives/Goals This purpose of this project is to sanitize tea of harmful microorganisms to make for safer consumption by immunocompromised persons. Methods/Materials Twenty 5 gram tea leaf samples were collected from tea bags and UV irradiated for varying amounts of time. Half of the samples were agitated as they were UV irradiated. The UV irradiated tea samples were plated on Sabouraud agar plates and placed in an incubator. Once a day, the plates were inspected to see if any mold spores appeared. Once I proved that UV light sanitizes tea of harmful microorganisms, I created a device that could sanitize larger quantities of tea. The experiment was repeated using the device, rather than UV treating each sample by hand. Each sample was plated, incubated, and inspected for spores. Results Black tea was irradiated for various time periods and plated on agar plates to see if UV light sanitizes black tea of harmful microorganisms. Half the tea was agitated as it was irradiated. The black tea that was agitated while being UV irradiated showed a significant decrease in harmful microorganisms. UV irradiation and agitation for over thirty-five minutes result in the sanitation of black tea of harmful microorganisms. Conclusions/Discussion My hypothesis that UV irradiation and agitation would sanitize black tea of harmful microorganisms was strongly supported by the results. I plan to extend my project and patent a device that will sanitize tea in individual portions for consumption by immunocompromised persons. Black tea was used in this experiment, but UV light also sanitizes other food products such as pepper. The findings of this experiment demonstrate a concrete way to improve the quality of life for immunocompromised persons by expanding their dietary options.
Summary statement
My experiment shows that UV light can be used to sanitize food products of microorganisms harmful to immunocompromised persons.
Help received
I designed and conducted the experiment myself. However, I was invited to conduct my experiments in the lab of Dr. Maria Elena DeBellard at CalState Northridge.
Competition history
- CSEF 2016
Resources
Related projects
CSEF · 2012
Portable Milk Purification System
CSEF · 2006
Using Solar Energy to Make Drinking Water Safe
CSEF · 2008
Cleaner Kitchen and Bath with UV Light
ISEF · 2021
UVClean or SODISgusting: Comparing the Effectiveness and Efficiency of an Ultra-Violet C (UVC) Sanitizer Box and the Solar Water Disinfection (SODIS) Method
ISEF · 2016
To Drink or Not to Drink: The Lysis of Escherichia coli by Ultraviolet Light through a Makeshift Filtration System
ISEF · 2017
To Drink or Not to Drink: The Lysis of Escherichia coli by Ultraviolet Light through a Makeshift Filtration System, Part Two
CSEF · 2012
Ultraviolet Light vs. Bacteria on Toothbrush
CSEF · 2008
Examining the Cleaning Effects of Practical Household Solutions on Produce
Closest projects by meaning, across every fair and year in the corpus.
Browse more like this
Source: California Science & Engineering Fair public projects