Protein-Specific Inhibition on the Multixenobiotic Resistance Mechanism of Daphnia Magna

Overview

Inhibitors of multixenobiotic resistance (MXR), a mechanism that protects aquatic organisms from pollutants, pose a danger to biological and ecological health. Inhibitors target individual proteins to prevent efflux of pollutants, although leaving any proteins uninhibited may allow MXR to function. However, the severity of protein-specific inhibition has not been addressed. Water flea Daphnia magna possesses proteins ABCB1 and ABCC, which are inhibited by Reversin 205 and MK571, respectively. The inhibitors were administered individually or combined at concentrations of 1 µM, 5µM, and 10µM to individual daphnids. Rhodamine B, a fluorescent substrate of MXR proteins, was also administered and its fluorescence was measured as a proxy for MXR function. The Reversin 205 and combined inhibitor treatments inhibited MXR significantly more compared to control and MK571 treatments, with higher concentrations showing greater inhibition, although the Reversin 205 and combined groups did not differ among themselves. Since MK571 did not differ significantly from control, ABCB1 appears to be more involved in D. magna’s MXR. The results suggest inhibitors of MXR may vary in severity across species. The influence an MXR protein has on the MXR function in specific organisms should be addressed to identify the risk an inhibitor poses in an ecological context.

Competition history

• AJAS 2018 Category not listed

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