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Plasmid DNA Extraction

CSEF · 2008 Biochemistry/ Molecular Biology

Niraj R. Patel · Centennial High School, Corona, Riverside County

Overview

Objectives/Goals The purpose of this project is to understand and determine the effects and factors that detergent solutions have on extracting pDNA from Escherichia coli. Methods/Materials Centrifuge Machine- Spins ependorf tubes to pellet bacteria of large wasteful chunks besides DNA gel electrophoresis tank- Measures the quality of extracted DNA spectrophotometer- Measures the quantity of extracted DNA Results The best extraction solutions were based of of concentration such as the Palmolive solution. Palmolive is a dish washing soap and was able to break through the cell membrane to to its characteristics. Conclusions/Discussion Palmolive and Pantene extracted the purest and most pDNA for Escherichia coli due to the concentration and thickness of the solutions.

Summary statement

The purpose of this project is to determine the most reliable and accurate solution for extractions

Help received

Kunal Jariwala (cancer researcher)- Allowed me to use lab and equipment

Competition history

  • CSEF 2008 Biochemistry/ Molecular Biology · Entry S0415

Resources

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