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PatchPal: A Trypsin-Responsive Hydrogel Eczema Patch for Targeted Ceramide Delivery

CSEF · 2026 Biochemistry/ Molecular Biology (Senior Division)

Saria Lum

Overview

Eczema, or atopic dermatitis, affects 30% of children and 10% of adults in the United States (NIH). While corticosteroids suppress inflammation, they weaken the skin barrier, causing recurrent flare-ups. Barrier-targeted treatments like supplementing ceramides show promise, yet dosing is inconsistent. To address this gap, I developed PatchPal, a hydrogel patch that provides barrier support through sustained hydration and a trypsin-responsive ceramide release. Patches were fabricated by mixing 2g sodium alginate, 100mL water, and 300mg ceramide-NP dissolved in 5mL 90% ethanol. 15-20mL was poured into petri dishes, solidified with 3-5 sprays 3% calcium lactate, and cut into 1’’x1’’ squares. Each patch was reinforced with 1% chitosan and a 10% gelatin protective layer that degrades with enzymatic activity. Across three 24-hour hydration trials, two patches gained up to 5.07% mass or retained moisture with minimal loss (-2.53%), while mechanical testing demonstrated lateral pull strengths of 11.96N-17.02N, confirming durability. In a trypsin-response test, patches dyed with 100μL methylene-blue were suspended in 6mL trypsin and monitored at 1, 3, 6, 12, and 24 hours. Increased blue intensity and absorbance indicated ceramide release, with 3.572×10⁻⁵mol/L (≈ 20mg, 6.85% of initial load) released over 24 hours: a value consistent with other hydrogel-based patches. Using a 4mm porcine gelatin barrier, colorimetric analysis showed the blue-value shifted from 195→32 (-83.5%), confirming substantial diffusion. Ultimately, these results demonstrate PatchPal’s hydration, ceramide release capabilities, and mechanical integrity: advancing eczema treatment from passive to responsive and reducing flare-ups, one patch at a time.

Competition history

  • CSEF 2026 Biochemistry/ Molecular Biology (Senior Division) · Entry S-04-11

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