Micropropagation of Explants from Mature Tissue of Quercus lobata

Overview

Objectives/Goals This study was undertaken to evaluate the optimum protocol for micropropagation using 1-year-old and 2-year-old plant tissue for the regeneration of valley oak in vitro. Methods/Materials Acorns were collected and germinated under clinical conditions during the fall of 2005, 2006, and 2007. Stem segments with a single axillary bud were obtained from six-month-old,one-year-old, and 2-year-old the valley oak seedlings. Each explant was cut to a length of approximately 1 cm, retaining the axillary bud, and placed in a petri dish containing 35 ml of Lloyd and McCown culture medium. The number of shoots on each explant was recorded after six and nine weeks. Statistical analyses were completed with the Statistical Analysis System. Results To investigate the germination of the Valley Oak qualitative accounts of the explants# growth were taken along with a quantitative analysis of shoot numbers. The initial initiation for each group tested tissue (6-month old tissue; 1 year old tissue; and 2-year old tissue) began with 6 explants each. As suspected the immature tissues of the six month old nodule segments appeared to be the healthiest and were easily sub-cultured. From initiation through four separate sub-cultures the 6-month old tissue produced 36 shoots in total (Fig. 2) This was due in fact to a large amount of shoot growth, although explants were either successful or were overtaken by contamination. While the one year explants did germinate and produce healthy stems their numbers were not as great as the six month old explants in that we began with 6 explants and ended up with 18 sub-cultured shoots. Oddly, the two year nodules survived and thrived. They produced 122 shoots by the end of the fourth sub-culturing. Conclusions/Discussion In conclusion, the data supported the hypothesis that the Lloyd & McCown Woody Plant Basal Medium with Vitamins will be an effective medium to use in the micropropagation of tissue ranging in age of 1 and 2-year-old of Valley Oaks (Quercus).

Awards (1)

• Honorable Mention

Competition history

• CSEF 2008 Plant Biology · Entry S1703

Resources

• CSEF project summary ↗

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